The E. coli pET expression system revisited—mechanistic correlation between glucose and lactose uptake
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چکیده
منابع مشابه
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We present a mathematical model of glucose-lactose diauxic growth in Escherichia coli including both the positive and negative regulation mechanisms of the lactose operon as well as the inducer exclusion. To validate this model, we first calculated the time evolution of βgalactosidase for only the lactose nutrient and compared the numerical results with experimental data. Second, we compared th...
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The effects of lactose, galactose, glucose, and glucose combined with galactose on the induction of β–galactosidase by IPTG in Escherichia coli strain VC10, growing in a glycerol media, were compared against a control of glycerol plus IPTG. After inducing β– galactosidase production with the IPTG, the cultures were altered with the addition of one of the above carbohydrates or combinations. Thi...
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Glucose-lactose diauxie in Escherichia coli.
Growth of Escherichia coli in medium containing glucose, at a concentration insufficient to support full growth, and containing lactose, is diauxic. A mutation in the gene, CR, which determines catabolite repression specific to the lac operon, was found to relieve glucose-lactose but not glucose-maltose diauxie. Furthermore, a high concentration of lactose was shown to overcome diauxie in a CR(...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2016
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-016-7620-7